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一般說明
β-半乳糖苷酶是四聚体蛋白,分子量为465 kDa。由 LacZ 基因编码。
應用
β-半乳糖苷酶可用于衍生化,例如 IgG 的酶标记,无需事先透析或凝胶过滤。
β由 大肠杆菌 提取的-半乳糖苷酶可用于在 B-390封装器(BUCHI)上产生生物催化性的 Ca-藻酸盐珠。 也用于制备原液滴溶液,还可作为 Pep-1(KETWWETWWTEWSQPKKKRKRKV-半胱酰胺)载体。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
β-半乳糖苷酶可视为报告酶,用于涉及基因表达调节、蛋白功能/结构分析和靶基因表达的各种应用。
包裝
基于蛋白质含量的包装尺寸
物理性質
四聚体的分子量为465 kDa(每个亚基116.3 kDa)
單位定義
一个单位将在 pH7.3,37°C 下每分钟将 1.0μ 摩尔邻硝基苯基 β-D-吡喃半乳糖苷水解成邻硝基苯酚和 D-半乳糖。
外觀
用磷酸盐缓冲液和蔗糖稳定
基底
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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