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包裝
pkg of 1 vial
運輸包裝
ambient
儲存溫度
−20°C
一般說明
The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA
應用
Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology
特點和優勢
HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.
外觀
Dry
準備報告
Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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实验方案
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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