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D4551

Sigma-Aldrich

N,N-二甲基甲酰胺

for molecular biology, ≥99%

别名:

DMF, NSC 5356

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About This Item

线性分子式:
HCON(CH3)2
CAS号:
68-12-2
分子量:
73.09
Beilstein:
605365
EC號碼:
200-679-5
MDL號碼:
MFCD00003284
分類程式碼代碼:
12352111
PubChem物質ID:
24893883
NACRES:
NA.52

等級

for molecular biology

品質等級

200

蒸汽密度

2.5 (vs air)

蒸汽壓力

2.7 mmHg ( 20 °C)

化驗

≥99%

形狀

liquid

自燃溫度

833 °F

expl. lim.

15.2 %

雜質

≤0.5% water
water

折射率

n20/D 1.430 (lit.)

bp

153 °C (lit.)

mp

−61 °C (lit.)

密度

0.944 g/mL (lit.)

適合性

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

儲存溫度

room temp

SMILES 字串

[H]C(=O)N(C)C

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

InChI 密鑰

ZMXDDKWLCZADIW-UHFFFAOYSA-N

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相关类别

一般說明

N,N-二甲基甲酰胺（DMF）是一种具有低蒸发速率的溶剂，可用于制备带有各种疏水性有机化合物的溶液，用于分子生物学应用。

應用

N,N-二甲基甲酰胺在细胞活力检测中用于溶解MTT晶体。它还用于在具有高酶活性的霉菌中对阿魏酸酯酶活性进行测定。
多种疏水性有机化合物的溶剂。

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

135.5 °F - closed cup

閃點（°C）

57.5 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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实验方案

cAMP Assays

cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.

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