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化驗
≥98% (HPLC)
形狀
powder
反應適用性
reaction type: Biotinylations
顏色
white to off-white
mp
212 °C
溶解度
DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)
DMSO: soluble
儲存溫度
−20°C
SMILES 字串
[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2
InChI
1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChI 密鑰
YMXHPSHLTSZXKH-RVBZMBCESA-N
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應用
(+)-生物素NHS酯，或称(+)-生物素 N-羟基琥珀酰亚胺酯，已用于：
- 生物素化Hyb 246-4，用于表面活性剂蛋白 D 酶联免疫吸附试验（SP-D ELISA）
- 生物素化蛋白质以形成预成型络合物
- 标记和修饰单克隆抗微纤维相关蛋白 4 (MFAP4) (HG-HYB 7-18)，以结合到alphaLISA 链霉亲和素涂层供体珠
生化／生理作用
N-羟基琥珀酰亚胺酯（NHS）生物素修饰有助于目标赖氨酸残基在ε-氨基上的生物素化，并识别表面赖氨酸残基。它被用作识别蛋白质与几种生物分子相互作用的工具，如蛋白质-多糖相互作用、蛋白质-蛋白质相互作用、蛋白质-核酸相互作用和蛋白质-配体相互作用。NHS-生物素可用于蛋白和肽的生物素化。它通常在6.5-8.5的pH范围内通常偶联至伯胺。
免責聲明
除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Helle Wulf-Johansson et al.
PloS one, 8(12), e82243-e82243 (2013-12-19)
Microfibrillar-associated protein 4 (MFAP4) is located in the extracellular matrix (ECM). We sought to identify tissues with high levels of MFAP4 mRNA and MFAP4 protein expression. Moreover, we aimed to evaluate the significance of MFAP4 as a marker of cardiovascular
M J Kim et al.
Analytical biochemistry, 231(2), 400-406 (1995-11-01)
Homogeneous-type enzyme-linked competitive binding assays utilizing the synthetic enzyme-biotin and avidin-riboflavin conjugates are developed for the detection of riboflavin as well as its binder protein. The activity of the enzyme-biotin conjugate is inhibited in the presence of the avidin-riboflavin conjugate
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Heparanase, an endo-glucuronidase that specifically cleaves heparan sulfate (HS), is upregulated in several pathological conditions. In this study, we aimed to find a correlation of heparanase expression and platelets production. In the transgenic mice overexpressing human heparanase (Hpa-tg), hematological analysis
Faqing Huang et al.
RNA (New York, N.Y.), 9(12), 1562-1570 (2003-11-19)
Expanding our previous finding of an adenosine-initiated transcription system, we now demonstrate that either the 5' site or the N6 site of adenosine nucleotides can be modified extensively without abolishing their ability to initiate transcription under the T7 phi2.5 promoter.
Erdem D Tabdanov et al.
STAR protocols, 1(1) (2020-08-01)
We present a reproducible protocol for fabrication of polyacrylamide (PAA) hydrogel-based nano-patterns and nano-textures with a wide range of elastic rigidities to study fundamental cell behaviors, such as mechanosensitivity and motility. We explore the benefits of this protocol by successfully
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