PG

95070629, fish ovary, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• 产品名称: PG, 95070629
• 生物源: fish ovary
• 包裝: tube of 5 μg 95070629-DNA-5UG; pkg of vial of cells 95070629-1VL
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Fish Northern Pike ovaries

細胞系描述

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

應用

Fish Virology

培養基

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

其他說明

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