CLC

95070628, fish blood, Epithelial-like

• 分類程式碼代碼: 41106514

属性

• 产品名称: CLC, 95070628
• 生物源: fish blood
• 包裝: tube of 5 μg 95070628-DNA-5UG; pkg of vial of cells 95070628-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 104
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Carp leukocyte

細胞系描述

The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2 year old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.

應用

Immunological research, fish virology, fish cell physiology.

培養基

EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Spilt confluent cultures 1:3 using 0.25% trypsin or trypsin/EDTA; 25-28°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

其他說明

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