EPC

93120820, fish skin, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: EPC, from fish skin, 93120820
• 生物源: fish skin
• 增長模式: Adherent
• 染色體組型: 2n = 96
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

说明

細胞系來源

Fish epithelioma Fathead minnow Pimephales promelas

細胞系描述

This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.

DNA分析

Not specified

培養基

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

其他說明

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