NB4 1A3

89121405, mouse nerve, Neuroblast

• 分類程式碼代碼: 41106514

属性

• product name: NB4 1A3, 89121405
• 生物源: mouse nerve
• 增長模式: Adherent
• 染色體組型: Unstable
• 形態學: Neuroblast
• 產品: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C-1300 Neuroblastoma

細胞系描述

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

應用

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

培養基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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