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product name
BW5147.G.1.4.OUAR.1, 88100507
生物源
mouse blood
增長模式
Suspension
染色體組型
Not specified
形態學
Lymphoblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse AKR/J T cell lymphoma
細胞系描述
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100βM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
應用
Resistance and hybridisation studies
培養基
DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
其他說明
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