HFL1

89071902, human lung (fetal), Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: HFL1, 89071902
• 生物源: human lung (fetal)
• 增長模式: Adherent
• 染色體組型: 2n = 46, diploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human foetal lung fibroblast

細胞系描述

Derived from a 16-18 week old human foetus. Finite lifespan; capable of at least 57 population doublings.

應用

Virus studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17

培養基

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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