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product name
MiCL1 (S+L-), 86020603
生物源
mink lung
增長模式
Adherent
染色體組型
2n = 30
形態學
Fibroblast-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mink lung, Moloney murine sarcoma virus infected
細胞系描述
A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.
應用
Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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