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Merck
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主要文件

MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

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About This Item

分類程式碼代碼:
41106514

product name

MiCL1 (S+L-), 86020603

生物源

mink lung

增長模式

Adherent

染色體組型

2n = 30

形態學

Fibroblast-like

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mink lung, Moloney murine sarcoma virus infected

細胞系描述

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

應用

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

其他說明

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