SC-1

86060301

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse embryo
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse feral embryo

細胞系描述

Sensitive to both N- and B- trophic viruses. Cells exhibit a greater sensitivity than other mouse lines to MuLV strains and to a limited extent support the replication of xenotropic MuLV. This cell line has been mycoplasma eradicated at ECACC.

應用

Virus studies: N- and B- trophic viruses, MuLV

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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