AFRC MAC 77

85102262, Reactivity: Anti-Nitrate Reductase

• 分類程式碼代碼: 41106514

属性

• 生物源: Rat x Rat Hybridoma
• ImmunoDon: F344 rat spleen
• 骨髓瘤: Y3Ag1.2.3
• 反應: Anti-Nitrate Reductase
• 技術: cell culture | mammalian: suitable
• 同型: IgG2b
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Y3Ag1.2.3 x Rat F344 spleen

細胞系描述

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

免疫原

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

培養基

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

其他說明

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