H9C2 (2-1) Cell Line from rat

88092904, rat cardiovascular , Myoblast

• 别名: H9c2 Cells
• 分類程式碼代碼: 41106514

属性

• product name: H9C2 (2-1) Cell Line from rat, 88092904, BDIX heart myoblast
• 生物源: rat cardiovascular
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Myoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat BDIX heart myoblast

細胞系描述

Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.

應用

Differentiation studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.

其他說明

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