HT29 Cell Line human

91072201, human colon, Epithelial

• 别名: HT29 Cells
• 分類程式碼代碼: 41106514

属性

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: 2n = 46, hypertriploid
• 形態學: Epithelial
• 產品: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

應用

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

培養基

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

其他說明

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