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product name
EL4 Cell Line murine, 85023105, Lymphoblast from blood
生物源
mouse blood
增長模式
Suspension
染色體組型
2n = 39
形態學
Lymphoblast
產品
A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse ascites lymphoma lymphoblast
細胞系描述
Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).
應用
EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.
Tumourigenicity studies
培養基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.
其他說明
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