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Merck

C3389

Sigma-Aldrich

乙酰胆碱酯酶 来源于电鳗

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

别名:

AChE, 乙酰胆碱乙酰水解酶, 胆碱酯酶,乙酰基

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.77

種類

Type VI-S

品質等級

形狀

lyophilized powder

比活性

200-1,000 units/mg protein

分子量

280 kDa

成份

Protein, ≥45% biuret

溶解度

20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)

應用

diagnostic assay manufacturing

儲存溫度

−20°C

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一般說明

分子量: 280 kDa
等电点: 5.5
消光系数: E1%= 18.0(280 nm)

来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。

應用

来自sigma的酶已被用作评估阿斯巴甜代谢物对海马乙酰胆碱酯酶活性影响的参考。该酶还用于快速检测乙酰硫代胆碱氯化物的固定化研究中。

生化/生理作用

体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。

單位定義

一单位在37°C、pH 8.0条件下每分钟可将1.0 μ摩尔的乙酰胆碱水解成胆碱和醋酸盐 。

外觀

含 Tris 缓冲盐的冻干粉

準備報告

该酶可在20 mM Tris-HCl, pH 7.5中以1 mg/mL的浓度溶解并产生清亮的溶液。

分析報告

使用乙酰胆碱作为底物而获得的活性是丁基胆碱通过使用来自电鳗的乙酰胆碱酯酶而获得活性的30-100倍。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Dan Du et al.
Analytical and bioanalytical chemistry, 387(3), 1059-1065 (2006-12-23)
A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved
Jingming Gong et al.
Biosensors & bioelectronics, 24(7), 2285-2288 (2008-12-30)
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs)
Gabriela Villalta et al.
Plants (Basel, Switzerland), 10(6) (2021-07-03)
The essential oil (EO) of Salvia leucantha Cav. was isolated by steam distillation of the aerial parts collected in the South of Ecuador. Its physical properties were evaluated and the chemical composition of the oil was determined by GC-MS and
Makar Makarian et al.
Journal of molecular structure, 1247 (2022-03-01)
In an effort to develop new therapeutic agents to treat Alzheimer's disease, a series of donepezil-based analogs were designed, synthesized using an environmentally friendly route, and biologically evaluated for their inhibitory activity against electric eel acetylcholinesterase (AChE) enzyme. In vitro
Acetylcholinesterase, I. Large-scale purification, homogeneity, and amino Acid analysis.
W Leuzinger et al.
Proceedings of the National Academy of Sciences of the United States of America, 57(2), 446-451 (1967-02-01)

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