推荐产品
一般說明
CelLytic M是一种专有的去污剂溶液，专为从培养的哺乳动物细胞中提取有效的全细胞蛋白而设计。 它能够对悬浮细胞和贴壁细胞进行有效和快速的细胞裂解和蛋白质溶解。 裂解物可用于许多下游应用而无需对CelLytic M进行去除，例如报告基因测定、Western印迹/免疫沉淀、凝胶阻滞实验、磷酸酶测定、以及激酶测定。
特點和優勢
- 高效：效率比冻融、超声和其他产品最多高50％
- 非变性：不会干扰下游应用，如免疫沉淀、激酶和磷酸酶测定、报告基因检测、以及凝胶阻滞实验
- 方便：即用型试剂无需从培养板上刮下
- 快速：可在室温下快速裂解细胞。
數量
用于蛋白质提取的CelLytic M试剂的效率已经在但不限于HeLa、CHO、COS、HL-60、Jurkat、A431、PC-12和牛主动脉内皮细胞（BAEC）上进行了测试。
分析報告
106-107 悬浮细胞使用125μl CelLytic M 。 对于贴壁细胞，在100 mm平板中使用500-1,000μL；在35 mm平板中适用200-400μL。
法律資訊
CelLytic is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Moon Kyung Joo et al.
Journal of gastroenterology and hepatology, 31(10), 1717-1726 (2016-10-30)
The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Both gene
Liliana F Iannucci et al.
Scientific reports, 7(1), 2023-2023 (2017-05-19)
Nonalcoholic fatty liver disease (NAFLD) is a major health problem worldwide, and is often associated with lipotoxic injury, defective mitochondrial function, and insulin resistance. Thyroid hormones (THs) are important regulators of hepatic lipid metabolism. Among the THs, diiodothyronine (T
Filipe Cabreiro et al.
Free radical biology & medicine, 51(8), 1575-1582 (2011-08-16)
The superoxide free radical (O(2)(•-)) has been viewed as a likely major contributor to aging. If this is correct, then superoxide dismutase (SOD), which removes O(2)(•-), should contribute to longevity assurance. In Caenorhabditis elegans, overexpression (OE) of the major cytosolic
Wen-Tsung Huang et al.
Frontiers in pharmacology, 8, 902-902 (2018-01-13)
Intrinsic or acquired resistance to hormone therapy is frequently reported in estrogen receptor positive (ER
Vinay Kumar Tripathi et al.
Molecular neurobiology, 54(5), 3633-3651 (2016-05-22)
Expression of various cytochrome P450s (CYPs) in mammalian brain cells is well documented. However, such studies are hampered in neural/glial cells of human origin due to nonavailability of human brain cells. To address this issue, we investigated the expression and
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门