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C4482

Sigma-Aldrich

CelLytic ™ Y 细胞裂解试剂

For yeast cells

别名:

Yeast cell lysis reagent

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About This Item

分類程式碼代碼:
41116134
NACRES:
NA.56

品質等級

200

形狀

solution

一般說明

CelLytic Y 细胞裂解试剂具有通用性、无磷酸盐和非变性。为细胞裂解和蛋白溶解提供了有效的方法。维持免疫反应性或生物学功能的靶蛋白。方案简短，在室温下执行。无需极端条件或玻璃珠。仅使用 2.5-5 mL/g 酵母细胞。

應用

CelLytic ™ Y 细胞裂解试剂已用于裂解表达 DNA 修复、Rad 蛋白、 热休克蛋白 (ssa1) 和人白细胞介素 4 (hIL4) 的酵母细胞。

特點和優勢

  • 快速：在 20 min 内实现细胞裂解
  • 效率：比玻璃珠高三倍
  • 柔性：加入还原剂 (DTT) 后裂解增强
  • 温和：非变性-保留生物活性

法律資訊

CelLytic is a trademark of Sigma-Aldrich Co. LLC

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319,H412

危險分類

Aquatic Chronic 3 - Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

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访问文档库

DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)

商品

Yeast Cell Lysis Reagents

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains.

相关内容

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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