推荐产品
形狀
liquid
製造商/商標名
Novagen®
儲存條件
OK to freeze
運輸包裝
wet ice
一般說明
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
特點和優勢
- Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods
成分
•1 ml or 5 ml100X THP Solution
警告
其他說明
法律資訊
訊號詞
Warning
危險聲明
危險分類
STOT RE 2 Oral
標靶器官
Kidney
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°C)
Not applicable
商品
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
相关内容
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
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