Whole Cell Lysis Reagents & Enhancers
Isolation and purification of proteins requires disruption of cells or tissue samples and extraction of the relevant protein fraction. This step is critical as processing methods can affect the target protein’s integrity and activity, or otherwise expose it to degradative conditions. A variety of reagents and enzymes for cell lysis and protein extraction provide an array of options tailored for specific cell types and application needs.
Related Product Resources
Brochure: Refine Protein Preparation - Tools for Better Protein Analysis
RIPA buffer
Radioimmunoprecipitation assay (RIPA) buffer is used for rapid cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. RIPA buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with immunoreactivity and biological activity. RIPA buffer also minimizes non-specific protein binding interactions to ensure low background in immunoprecipitation and molecular pull-down assays.
BugBuster® protein extraction reagents
BugBuster® protein extraction reagents are optimized for protein extraction from E. coli under non-denaturing conditions.
BugBuster® Master Mix combines the BugBuster® bacterial protein extraction reagent with Benzonase® nuclease and rLysozyme™ solution for convenient, all-in-one protein extraction and nucleic acid digestion.
- YeastBuster™ protein extraction reagents are available for protein extraction of yeast cultures.
- CytoBuster™ protein extraction reagent is available for lysis and protein extraction from insect and mammalian cells.
- NucBuster™ reagent enables extraction of nuclear proteins in less than 30 minutes with a simple 2-step protocol.
- PhosphoSafe™ extraction reagent includes phosphatase inhibitors to preserve phosphorylation state of sample proteins.
CelLytic™ lysis reagents
CelLytic™ lysis reagents are specifically formulated to lyse and extract cellular proteins under non-denaturing conditions based on the cell or tissue type and target protein localization.
PopCulture® reagents
PopCulture® reagents enable protein extraction from cells directly in the culture medium with no centrifugation required. Insect PopCulture® reagent lyses insect cells directly in serum-free medium and is ideal for expression screening of many small samples.
Enhancers: Extraction enzymes and other reagents
- rLysozyme™ solution: Enzymatically cleaves bonds in the peptidoglycan layer of bacteria to break through the cell wall for lysis or spheroplast prep, especially effective against gram positive species
- Benzonase® nuclease: degrades all types of nucleic acid for more efficient protein extraction, to reduce viscosity and assay interference allowing for accurate sample handling and faster chromatography
- Lysonase™ bio-processing reagent: convenient mixture of rLysozyme™ solution and Benzonase® nuclease minimizes pipetting steps
- Stabilyser™ reagent: stabilizes functional protein and maintains nucleic acid integrity; prevents degradation during tissue lysis and storage
- Lyticase enzyme: enzymatically cleaves yeast cell wall β-glucan layers allowing for access to cell contents. Digestion of the outer layer helps prepare spheroplasts for lysis or transformation
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