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一般說明
抗-V5 琼脂糖亲和凝胶由与溴化氰活化的珠状琼脂糖偶联的单克隆抗-V5 抗体组成。 该抗体识别转染的哺乳动物细胞中表达的 V5 标记的重组融合蛋白。
抗-V5琼脂糖亲和凝胶由纯化的单克隆抗-V5抗体免疫球蛋白部分与溴化氰活化的珠状琼脂糖偶联组成,单克隆抗-V5抗体从 V5-10杂交瘤腹水中分离。纯化的单克隆抗体固定在琼脂糖上。
特異性
识别 V5 标签(GKPIPNPLLGLDST)融合蛋白
應用
鼠源抗V5琼脂糖亲和凝胶抗体用于免疫沉淀和重组蛋白纯化。
生化/生理作用
重组 DNA 技术可将目的基因附着在特定序列或基因上,提供′亲和柄′设计,选择性识别和纯化目的蛋白。 在给定基因中添加V5标签序列(GKPIPNPLLGLDST)会产生稳定的融合产物,这一产物似乎不会干扰蛋白的生物活性或干扰标记产物的生物分布。研究人员已经设计出许多重组蛋白表达衍生自猴副流感病毒V5的短序列,这些短序列称为V5-标签。抗“标签”琼脂糖结合物可以促进检测、分离和纯化这些蛋白质。
外觀
将珠状琼脂糖(1:1v / v)悬浮于含有 15mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水中。
儲存和穩定性
为保证连续使用和延长存储时间,请在2°C至8°C条件下存储。请勿冷冻。
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除非产品目录中另有说明,否则我们的产品仅供研究使用,不得在人类(或动物)上用于任何其他目的,包括但不限于未经授权的商业使用、体外诊断、离体(或体内)治疗或进行任何类型的摄入或应用。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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