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A4596

Millipore

ANTI-FLAG® M1琼脂糖亲和凝胶

别名:

抗-ddddk, 抗-dykddddk

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.32

共軛

agarose conjugate

品質等級

200

抗體產品種類

primary antibodies

形狀

suspension

同型

IgG12b

容量

≥0.6 mg/mL, gel binding capacity

儲存溫度

−20°C

相关类别

一般說明

ANTI-FLAG®M1琼脂亲和凝胶是一种纯化小鼠IgG2B单克隆抗体共价连接到琼脂糖上。

特異性

结合特异性：FLAG 序列
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端

應用

用于纯化N末端FLAG融合蛋白。由于结合过程不依赖Ca2+，蛋白质能采用含EDTA的缓冲液洗脱，也可以使用FLAG多肽或甘油-HLC缓冲液(pH3)通过标准方法洗脱。ANTI-FLAG M1不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未加工处理的细胞质表达融合蛋白。

该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。

免疫沉淀(IP)：适用

洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA

更多产品信息请访问我们的FLAG®应用页面。

特點和優勢

  • 通常从粗裂解物中纯化融合蛋白，然后只使用一步层析，纯化成单条带
  • 用EDTA温和洗脱，将融合蛋白从亲和树脂上洗脱下来。
  • FLAG多肽溶液可以用于FLAG融合蛋白温和地，非变性洗脱。

外觀

球形琼脂糖悬浮在含10 mM磷酸钠、150mM NaCl (pH7.4)、0.02% (w/v) 叠氮化钠的50%甘油溶液中

其他說明

ANTI-FLAG® M1并不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未处理的细胞质表达融合蛋白。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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