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Merck

F3040

Sigma-Aldrich

Anti-FLAG® M1小鼠单抗 小鼠抗

clone M1, purified immunoglobulin, buffered aqueous solution

别名:

抗 ddddk, 抗 dykddddk

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.32

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

M1, monoclonal

形狀

buffered aqueous solution

純化經由

using Protein A

物種活性

all

濃度

2-5 mg/mL

技術

western blot: 10 μg/mL

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。

纯化方法-蛋白A

免疫原

FLAG;肽序列 DYKDDDDK

應用

小鼠产生的单克隆抗-FLAG® M1 抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹或EIA 。

成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀(1 篇文章)
免疫荧光(1 篇文章)

请访问我们的FLAG® 应用门户网站,了解更多产品详情。

生化/生理作用

当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合,因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性;该复合物在不存在钙离子的情况下解离。

外觀

溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。

準備報告

将抗体稀释至 10ug/mL,置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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