推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
M5, monoclonal
形狀
buffered aqueous solution
純化經由
using Protein A
物種活性
all
濃度
2-5 mg/mL
技術
western blot (chemiluminescent): 10 μg/mL
同型
IgG1
免疫原序列
DYKDDDDK
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
单克隆 ANTI-FLAG ® M5 是一种小鼠抗体，可与 N-末端 Met-FLAG 融合蛋白结合。可用于检测哺乳动物和果蝇细胞中表达的 N-末端 Met-FLAG 融合蛋白。
纯化方法-蛋白A
纯化方法-蛋白A
免疫原
FLAG；肽序列 DYKDDDDK
應用
小鼠生产的单克隆抗FLAG®M5抗体已被用于免疫印迹和免疫荧光。
只有当 FLAG 肽位于甲硫氨酸前面的氨基末端时，才能与其结合。结合不依赖于 Ca2+。可用于检测哺乳动物细胞粗提物中细胞质表达的 Met-FLAG 融合蛋白，但不推荐用于在大肠杆菌中表达的融合蛋白。
外觀
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
準備報告
用 Tris 缓冲盐水 (TBS) 将抗体稀释至 10 mg/mL：0.05 M Tris，0.15 M NaCl，pH 7.4。
其他說明
抗体不具有钙依赖性。
法律資訊
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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