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Merck

A0157

Sigma-Aldrich

抗坏血酸氧化酶 来源于南瓜

lyophilized powder, 1,000-3,000 units/mg protein

别名:

L-抗坏血酸:氧氧化还原酶, 抗坏血酸酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

plant (Cucurbita spp.)

品質等級

形狀

lyophilized powder

比活性

1,000-3,000 units/mg protein

儲存溫度

2-8°C

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一般說明

抗坏血酸氧化酶是一种同二聚体酶,每个亚基包含552个氨基酸残基(西葫芦)。每个亚基对应的分子量为70 kDa。这种酶主要存在于植物、真菌和真细菌中。

應用

西葫芦来源抗坏血酸氧化酶可用于:
  • 作为在培养基中分化成成骨细胞的补充剂
  • 在单脱氢抗坏血酸还原酶分析中氧化抗坏血酸产生单脱氢抗坏血酸
  • 测定抗坏血酸(AsA)和脱氢抗坏血酸(DHA)浓度

来自南瓜( Cucurbita sp.)的抗坏血酸氧化酶可用于研究氧化应激和热应激反应以及耐受性。 Sigma的抗坏血酸氧化酶已被用于抗坏血酸测定,以研究拟南芥( Arabidopsis的热应激反应。

生化/生理作用

抗坏血酸氧化酶(AO)可通过调节质外体的还原/氧化(氧化还原)参与细胞生长。这种酶能在质外体中合成氧化分子脱氢抗坏血酸(DHA)。此外,还参与了细胞延长和增大发育。
抗坏血酸氧化酶可将抗坏血酸转化为脱氢抗坏血酸。 它对L-抗坏血酸和一些类似物具有高度特异性。 抗坏血酸氧化酶是分子量约140kda的二聚体。

單位定義

在pH 5.6、温度25℃条件下,每分钟将1.0 μm的 L-抗坏血酸盐氧化成脱氢抗坏血酸盐所需的酶量为一个单位。

外觀

冻干粉末,含有缓冲液和蔗糖作为稳定剂。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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