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SCR011

Sigma-Aldrich

B6-White Murine ES Cell Line

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.

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About This Item

分類程式碼代碼:
41106509
eCl@ss:
32011203
NACRES:
NA.81

生物源

mouse

品質等級

製造商/商標名

B6-White

技術

cell culture | stem cell: suitable

輸入

sample type: mouse embryonic stem cell(s)

運輸包裝

liquid nitrogen

一般說明

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function. The influence of genetic background on phenotype has been shown to be an important consideration in selection of a mouse model. Furthermore, the time required to achieve congenic status, which can be 18-24 months has further stimulated demand for targeting in pure inbred strains including C57BL/6 (B6) mice.

Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.

For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).

STRAIN:C57BL/6-tyr(c-2J) albino mice

GENDER: Male

KARYOTYPE: 40XY

細胞系描述

Embryonic Stem Cells

應用

Research Category
Stem Cell Research

外觀

Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO

儲存和穩定性

Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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