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Merck
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Key Documents

MSIPS4W10

Millipore

Multiscreen® 96孔板,疏水PVDF薄膜

pore size 0.45 μm, sterile

别名:

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

分類程式碼代碼:
41104923
eCl@ss:
32039006
NACRES:
NB.22

材料

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

品質等級

描述

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

無菌

sterile

產品線

MultiScreen®

特點

hydrophobic
lid

製造商/商標名

MultiScreen®

參數

50-250 μL sample volume (per well)

技術

ELISPOT: suitable

過濾面積

0.28 cm2

板尺寸

96 wells

孔最大容積

300 μL

有效容積

50-250 μL

基質

Immobilon®-P

孔徑

0.45 μm pore size

結合類型

high binding surface

運輸包裝

ambient

一般說明

聚偏氟乙烯(PVDF)滤膜板胜过混合纤维素酯膜,对捕获抗体的结合能力略有提高,斑点检测性能优异,尤为适合荧光法应用。

應用

MultiScreen® HTS IP过滤板(0.45 μm,白色,无菌)已用于下列细胞的酶联免疫斑点法(ELISpot)分析:
  • 产双链DNA的浆细胞
  • 记忆B细胞和骨髓长寿浆细胞
  • 引流淋巴结和脾脏细胞

法律資訊

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain

商品

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

实验方案

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

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