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Key Documents

MSIPS4510

Millipore

Multiscreen® 96孔板,疏水PVDF薄膜

pore size 0.45 μm, sterile

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About This Item

分類程式碼代碼:
41104923
eCl@ss:
32039006
NACRES:
NB.22

材料

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

品質等級

描述

Sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

無菌

sterile

產品線

MultiScreen®

特點

hydrophobic
lid

製造商/商標名

MultiScreen®

參數

50-250 μL sample volume (per well)

技術

ELISPOT: suitable

過濾面積

0.28 cm2

板尺寸

96 wells

孔最大容積

300 μL

有效容積

50-250 μL

基質

Immobilon®-P

孔徑

0.45 μm pore size

輸入

sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)

結合類型

high binding surface

運輸包裝

ambient

一般說明

无论您’的应用是什么,密理博都为满足您的具体应用需求开发出适用的过滤板。MultiScreen过滤板长期以来一直都是促进药物研发和生命科学研究的可靠工具。

HTS过滤板(以MS开头的板目录号)
- MultiScreenHTS过滤板专为自动工作站的大呑吐量应用而开发。坚固的侧壁更易于使用,充裕的表面提供了贴条形码标签的地方。每个板孔都单独密封,防止培养基互扰,而且下排水是可以取下的,从那里可以接触到滤膜。过滤板有96孔和384孔平台,具有广泛的膜和塑料选择。

- Millipore’s的新型MultiScreen HTS+大流量过滤板设计减轻了非特异性结合,减小了背景噪音和信号强度的差异,尤其是对生化筛选测定。

传统过滤板(以MS开头的板目录号)
- 传统的多孔设计对要求将滤膜浸入测定皿的许多应用特别有价值。易于拆卸的下排水管和独特的板设计是Elispot、PAMPA、细胞筛选(迁移、侵袭或趋化性)或topcount检测用户等分析的理想选择。

配件 – MultiScreenHTS真空多联装置
- MultiScreenHTS真空多联装置专门设计用于与MultiScreenHTS过滤板配合使用。这一组合提供了优化的过滤特征以及无缝自动化整合。

Millicell测定板
- 除了传统的测定板,过滤板还有配装细胞培养皿的型号,用于培养和测定悬浮的和粘附的细胞系。在滤板上生长的细胞比在传统塑料基质上生长的细胞分化更好,从而产生更多的体内样结果。该滤板/托盘组合可用于多种细胞测定,包括转运、毒性和筛选测定。
滤器编码:知识产权

應用

无菌、无色96孔过滤板,配有孔径为0.45 um的疏水性PDVF滤膜,用于ELISPOT测定。每包10个。
研究类别
测定

细胞培养

外觀

色码:透明

其他說明

自动化兼容性:是

法律資訊

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs.
Ferrari, L, et al.
Research in Veterinary Science (2010)
Critical parameters in blood processing for T-cell assays: validation on ELISpot and tetramer platforms.
Afonso, Georgia, et al.
Journal of Immunological Methods, 359, 28-36 (2010)
One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions.
Martelli, Paolo, et al.
Veterinary Microbiology (2010)
Fang Chen et al.
Gastroenterology, 158(4), 1058-1071 (2019-12-07)
We investigated antibody responses to hepatitis C virus (HCV) antigens E1 and E2 and the relevance of animal models for vaccine development. We compared antibody responses to vaccination with recombinant E1E2 complex in healthy volunteers, non-human primates (NHPs), and mice.
Mariusz Skwarczynski et al.
Science advances, 6(5), eaax2285-eaax2285 (2020-02-18)
To be optimally effective, peptide-based vaccines need to be administered with adjuvants. Many currently available adjuvants are toxic, not biodegradable; they invariably invoke adverse reactions, including allergic responses and excessive inflammation. A nontoxic, biodegradable, biocompatible, self-adjuvanting vaccine delivery system is

商品

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

实验方案

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

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