MABN2431

Anti-phospho-NFL (Ser473) Antibody, clone 4F8

clone 4F8, from mouse

• 别名: Neurofilament light polypeptide, 68 kDa neurofilament protein, Neurofilament triplet L protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4F8, monoclonal
• 物種活性: human, mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μL
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_006149
• UniProt登錄號: P07196
• 目標翻譯後修改: phosphorylation (pSer473)
• 基因資訊: human ... NEFL(4747)

说明

一般說明

Neurofilament light polypeptide (UniProt: P07196; also known as NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein) is encoded by the NEFL (also known as NF68, NFL) gene (Gene ID: 4747) in human. Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and in adrenal and extra-adrenal pheochromocytomas. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Mutations in NEFL gene can lead to Charcot-Marie-Tooth disease 1F and 2E disease that is characterized by demyelinating and progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms.

特異性

Clone 4F8 specifically detects Neurofilament light polypeptide phosphorylated on Serine 473 in human and murine cells.

免疫原

KLH-conjugted linear peptide corresponding to 15 amino acids surrounding phosphorylated serine 473. Cystine was added for conjugation to KLH.

應用

Anti-phospho-NFL (Ser473), clone 4F8, Cat. No. MABN2431, is a highly specific mouse monoclonal antibody that targets Neurofilament light polypeptide phosphorylated on Ser473 and has been tested for use in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected phospho-NFL (Ser473) in Immunohistochemistry applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Western Blotting Analysis: A representative lot detected phospho-NFL (Ser473) in Western Blotting applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Research Category
Neuroscience

品質

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-NFL (Ser473) in mouse brain tissue lysate.

標靶描述

~70 kDa observed; 61.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.