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生物源
human
品質等級
化驗
>95% (electrophoresis)
形狀
liquid
製造商/商標名
Chemicon®
濃度
200 μg/mL
NCBI登錄號
UniProt登錄號
基因資訊
human ... ITGAV(3685)
一般說明
Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990].
Two main protein bands corresponding to alphaV (145 kDa) and beta3 (92 kDa) subunits are seen in silver stained polyacrylamide gels under non-reducing conditions. Minor protein band (170 kDa) corresponds to beta3 subunit disulfide dimer.
Two main protein bands corresponding to alphaV (145 kDa) and beta3 (92 kDa) subunits are seen in silver stained polyacrylamide gels under non-reducing conditions. Minor protein band (170 kDa) corresponds to beta3 subunit disulfide dimer.
應用
Electrophoresis and Immunoblotting control. Also useful for in vitro binding
experiments with metalloproteinase MMP-2 .
experiments with metalloproteinase MMP-2 .
外觀
Purified protein in 20 mM Tris-HCI, pH 7.5, 150 mM NaCI, 2 mM MgCI2, 0.2% Triton X-100. No preservatives.
儲存和穩定性
Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.
分析報告
7.5% SDS-PAGE by the method of Laemmli gives two major protein bands, corresponding to alpha V subunit (145 kDa) and beta 3 subunit (92 kDa) when visualized by silver stain (2-2.5 mg protein / lane nonreduced).A minor protein band (170 kDa) corresponds to the beta 3 dimer, similar to an occurrence of the beta 1 dimer upon purification of beta 1 integrins [Belkin et al., 1996].In some cases a beta 3 trimer is visible at 260 kDa.After reduction, with protein quantities of 1.5 - 4μg protein / lane, three major protein bands are apparent, corresponding to beta 3 integrin subunit (110 kDa), heavy alpha V chain (125 kDa) and light alpha V chain (25 kDa).A minor band at 200 kDa region is presumed to be the reduced beta 3 dimer, consistent with previous publications describing a 230 kDa beta 1 reduced dimer for beta 1 integrin purifications described previously.[Belkin et al.,1996].Faint bands at 80 kDa and 55 kDa correspond to minor degradation products of beta 3.CONTAMINANTS: Two faint bands identified at 210 and 220 kDa, which may correspond to the alpha V dimer.
Specific Activity: Functional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Biochemistry and molecular biology international, 40(1), 53-60 (1996-09-01)
Integrins of the beta 1 family were isolated from human smooth muscle. SDS-PAGE analysis and subsequent immunoblotting demonstrated that integrin samples contain a protein immunologically related to beta 1 integrin subunit with the previously undescribed apparent molecular mass 205 kD.
Cancer research, 65(24), 11545-11552 (2005-12-17)
The up-regulation of various matrix metalloproteinases (MMP), certain cell receptors such as integrins and CD44, and the SIBLING family of integrin-binding glycophosphoproteins have been reported separately and in various combinations for many types of tumors. The mechanisms by which these
American journal of physiology. Cell physiology, 283(5), C1501-C1510 (2002-10-10)
Thrombin has been reported to be a potent angiogenic factor both in vitro and in vivo, and many of the cellular effects of thrombin may contribute to activation of angiogenesis. In this report we show that thrombin-treatment of human endothelial
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