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一般說明
The pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag coding sequence that results in native protein after purification and cleavage. This plasmid carries an origin derived from RSF1030 (1, 2) and kanamycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors. Please read technical bulletin, TB401, for all possibilities and contact technical service if you need additional information.
This plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional S•Tag coding sequence is present at the distal end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (3).
This plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional S•Tag coding sequence is present at the distal end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (3).
警告
Toxicity: Standard Handling (A)
其他說明
1. Som, T. and Tomizawa, J. (1982) Mol. Gen. Genet.187, 375–383.
2. Cannon, P.M. and Strike, P. (1992) Plasmid27, 220–230.
3. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
2. Cannon, P.M. and Strike, P. (1992) Plasmid27, 220–230.
3. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
法律資訊
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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