71340-M

pCDFDuet-1 DNA - Novagen

• 别名: Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• 分類程式碼代碼: 41106602
• NACRES: NA.51

属性

• 形狀: liquid
• 品質等級: 100
• 製造商／商標名: Novagen^®
• 儲存條件: OK to freeze
• 啟動子: Promoter name: T7
• 選擇: spectinomycin
• 儲存溫度: −70°C

说明

一般說明

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

應用

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

警告

Toxicity: Standard Handling (A)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable