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Merck

T0699

Sigma-Aldrich

三氯乙酸 溶液

6.1 N

别名:

TCA

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About This Item

线性分子式:
Cl3CCOOH
CAS号:
分子量:
163.39
Beilstein:
970119
MDL號碼:
分類程式碼代碼:
12352106
PubChem物質ID:
NACRES:
NA.26

形狀

liquid

濃度

6.1 N
~100 % (w/v)

SMILES 字串

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI 密鑰

YNJBWRMUSHSURL-UHFFFAOYSA-N

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一般說明

三氯乙酸(TCA)是一种强酸。在饮用水pH值下,三氯乙酸呈现盐形式。

應用

三氯乙酸的应用示例:
  • 用于吲哚胺2,3-双加氧酶(IDO)活性测定,水解N-甲酰犬尿氨酸并生成犬尿氨酸
  • 用于人肺动脉平滑肌细胞(HPASMCs)增殖
  • 在蛋白提取和定量时,处理研磨组织和沉淀蛋白

生化/生理作用

三氯乙酸(TCA)全身性无毒,长久以来被用作浅表脱皮试剂。可用作过氧化物酶体增殖剂。
三氯乙酸溶液一般用于沉淀蛋白。可用于通过定量沉淀法测定蛋白浓度。三氯乙酸也可用作显微脱钙剂和固定剂。

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Guillaume Laflamme et al.
Cell reports, 26(11), 2875-2889 (2019-03-14)
The segregation of chromosomes is a critical step during cell division. This process is driven by the elongation of spindle microtubules and is tightly regulated by checkpoint mechanisms. It is unknown whether microtubules affect checkpoint responses as passive contributors or
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Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 18(1), 55-62 (2012-10-17)
The ratio of the nitric oxide synthase (NOS) cofactor tetrahydrobiopterin (BH(4)) to its oxidized form dihydrobiopterin (BH(2)) has been suggested as an index of endothelial dysfunction. Consequently, much effort has been put into preserving the in vivo equilibrium between these
Ying Wang et al.
Diabetes, 63(8), 2643-2655 (2014-03-13)
After diabetes, the heart has a singular reliance on fatty acid (FA) for energy production, which is achieved by increased coronary lipoprotein lipase (LPL) that breaks down circulating triglycerides. Coronary LPL originates from cardiomyocytes, and to translocate to the vascular
Verena Pfahler et al.
The New phytologist, 197(1), 186-193 (2012-10-31)
The objective of this study was to investigate the isotopic composition of oxygen bound to phosphate (δ(18)O-PO(4)) in different phosphorus (P) pools in plant leaves. As a model plant we used soybean (Glycine max cv Toliman) grown in the presence
Inga Müller et al.
PloS one, 9(5), e97285-e97285 (2014-05-27)
Members of the MYC family are the most frequently deregulated oncogenes in human cancer and are often correlated with aggressive disease and/or poorly differentiated tumors. Since patients with MYCN-amplified neuroblastoma have a poor prognosis, targeting MYCN using small molecule inhibitors

商品

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

实验方案

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

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