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70751

Millipore

BugBuster® Ni-NTA His•Bind®纯化试剂盒

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

製造商／商標名

Novagen®

儲存條件

OK to freeze

運輸包裝

wet ice

一般說明

BugBuster®Ni-NTA HIS-BIND®纯化试剂盒用于蛋白质纯化。Ni-NTA HIS-BIND®树脂是一种高性能的Ni2+带电琼脂糖，用于快速一步纯化含多组氨酸标签序列的蛋白质。

應用

BugBuster®Ni-NTA HIS-BIND®纯化试剂盒已用于His标记蛋白的纯化，如海藻糖-6-磷酸磷酸酶(TPP)、Ras(WT)蛋白和CsgA（主要curlin亚基）蛋白。

成分

•2 × 100 mlBugBuster 蛋白提取试剂

•10,000 U苯甲酸酯酶核酸酶，纯度> 90％

•10 mlNi-NTA His•结合树脂

•pkg/4色谱柱

警告

毒性：多个毒性值，请参阅 MSDS（O）

其他說明

由于该运输中有害物质的性质，您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系，以获取有关这些费用的更多信息。

法律資訊

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Flame
GHS02

訊號詞

Danger

危險聲明

H225

危險分類

Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 3

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Sagar Lahiri et al.
Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
Chae-Seok Lim et al.
Small (Weinheim an der Bergstrasse, Germany), 13(40) (2017-08-16)
Intermolecular interactions dominate the behavior of signal transduction in various physiological and pathological cell processes, yet assessing these interactions remains a challenging task. Here, this study reports a single-molecule force spectroscopic method that enables functional delineation of two interaction sites
Sarah A Tursi et al.
PLoS pathogens, 13(4), e1006315-e1006315 (2017-04-15)
Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via

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BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

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