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P6611

Millipore

HIS-Select® 镍亲和凝胶

(1:1 suspension in a 20% ethanol solution)

别名:

Ni-NTA resin, nickel charged agarose

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.56

共軛

magnetic beads

品質等級

200

形狀

(1:1 suspension in a 20% ethanol solution)

特點

hydrophilic

包裝

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

濃度

1.5-2.4 mL/mL (suspension in packed gel)

技術

protein purification: suitable

顏色

faint blue to very dark blue

基質

6% Beaded Agarose

容量

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

轉變溫度

flash point 32 °C (closed cup)

儲存溫度

2-8°C

相关类别

一般說明

HIS-Select®镍亲和凝胶强大耐用，可在高流速下捕获带组氨酸标签的重组蛋白。带组氨酸标签的重组蛋白可在天然或变性条件下结合。
HIS-Select®镍亲和凝胶是用于纯化组氨酸标签蛋白的固定化金属离子亲和色谱(IMAC)产品。特制四齿NTA螯合基团与镍琼脂糖珠之间特有的无电荷亲水性连接可确保在蛋白纯化中对小到中等大小的组氨酸标签蛋白呈现高选择性，同时又能降低与其他蛋白的非特异性结合。HIS-Select镍亲和凝胶对组氨酸标签蛋白具有选择性，对其他蛋白则只有较低的非特异性结合。选择性可通过在色谱过程中加入咪唑调节。

應用

HIS-Select®镍亲和凝胶用于纯化重组蛋白，如EF手型钙结合蛋白(S100A14)、 LIM同源框转录因子1α蛋白、sigma-1受体以及harpin蛋白、稳定蛋白1(SP1)和BCR-ABL融合蛋白。

特點和優勢

  • 带来更高纯度的高选择性。
  • 特有的非电荷亲水性连接减少非特异性结合。
  • 组氨酸标签蛋白结合容量超过15 mg/mL。
  • 在变性或非变性条件下都能结合。
  • 一步完成纯化。
  • 最大限度减少不必要的离子相互作用。
  • 极低的镍浸出。
  • 粒径：45-165 μm。

聯結

它也适用 EZview™ 技术（产品代码 E3528）。

外觀

20% 乙醇溶液的混悬液 (1:1)

儲存和穩定性

存放恰当的HIS-Select镍亲和凝胶至少可稳定保存一年。HIS-Select镍亲和凝胶在每次用后都应清洗，储存缓冲液中应添加20%乙醇等抗菌剂。

法律資訊

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

产品编号
说明
价格

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

89.6 °F - closed cup

閃點（°C）

32 °C - closed cup

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