推荐产品
製造商/商標名
Cytiva 28-4013-53
包裝
pkg of 50 x 100 μL
床體積
100 μL
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-14(Ni2+-stripped medium.)
工作範圍
3-12(Ni2+-stripped medium.)
容量
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
一般說明
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible.
特點和優勢
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose™ High Performance in prepacked spin columns.
- Yields up to 750 μg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
12 - Non Combustible Liquids
其他客户在看
商品
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
实验方案
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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