GE29-0005-94

His GraviTrap^™ TALON^®

Cytiva 29-0005-94, pack of 10 columns

• 别名: His GraviTrap
• 分類程式碼代碼: 23151816
• NACRES: NA.56

属性

• ligand: TALON^® Superflow Cobalt-IMAC
• 包裝: pack of 10 columns
• 製造商／商標名: Cytiva 29-0005-94
• 床體積: 1 mL
• 柱內徑: 15 mm
• 基質: 6% cross-linked agarose
• 粒徑: 60-160 μm
• cleaning: 2-14(Co²⁺-stripped medium)
• 工作範圍: 3-12(Co2+-stripped medium)
• 容量: ≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary); ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
• 儲存溫度: 2-8°C

说明

一般說明

His GraviTrap^™ Talon^® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

應用

His GraviTrap^™ Talon^® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap^™ Talon^® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon^® Superflow medium is negligible.

特點和優勢

• Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
• Ready-to-use gravity flow column prepacked with 1 mL Talon^® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• One run takes about 30 minutes, and large sample volumes can be applied all at once.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids