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616382

Sigma-Aldrich

Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride

Inhibits trypsin-like serine proteinases.

别名:

Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride, TLCK

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About This Item

经验公式（希尔记法）:
C14H21ClN2O3S · xHCl
CAS号:
4238-41-9
分子量:
332.85 (free base basis)
分類程式碼代碼:
12352200
NACRES:
NA.77

品質等級

100

化驗

≥95% (elemental analysis)

形狀

solid

製造商／商標名

Calbiochem®

儲存條件

OK to freeze
desiccated (hygroscopic)

顏色

off-white

溶解度

aqueous solution, pH <6.0: 1 mg/mL

運輸包裝

ambient

儲存溫度

2-8°C

InChI

1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1

InChI 密鑰

YFCUZWYIPBUQBD-ZOWNYOTGSA-N

一般說明

Inhibits trypsin-like serine proteases. Inactivates trypsin irreversibly without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Suggesting working concentration is 10-100 µM.
Inhibits trypsin-like serine proteinases. Irreversibly inactivates trypsin without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to the target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemia cells and normal thymocytes.

生化／生理作用

Cell permeable: no
EC50 = 80 µM blocking nitric oxide synthase induced by interferon-γ and lipopolysaccharides in macrophages
Primary Target
trypsin like proteases
Product does not compete with ATP.
Reversible: no

警告

Toxicity: Irritant (B)

重構

Unstable in solution; reconstitute just prior to use.

其他說明

Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Kim, H., et al. 1995. J. Immunol.154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol.22, 2547.
Bruno, S., et al. 1992. Leukemia6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol.41, 615.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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