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生物源
human
品質等級
形狀
liquid
儲存期限
6 mo.
分子量
Mw 35 kDa (cdk1)
Mw 75 kDa (cyclin B)
製造商／商標名
Upstate®
技術
activity assay: suitable (kinase)
溶解度
water: soluble
NCBI登錄號
UniProt登錄號
儲存溫度
−70°C
基因資訊
human ... CCNB1(891) , CDK2(891)
一般說明
Research area: Apoptosis
Cyclin dependent kinase 1 (CDK1), a type of serine/threonine kinase, is a catalytic subunit of the M phase-promoting factor (MPF) complex.
Cyclin dependent kinase 1 (CDK1), a type of serine/threonine kinase, is a catalytic subunit of the M phase-promoting factor (MPF) complex.
生化／生理作用
Cyclin B protein regulates mitosis in all eukaryotes. Cyclin B binds to cyclin dependent kinase 1 (CDK1), forming a complex that triggers the start of mitosis by phosphorylating specific proteins. Cyclin B regulates the activation, subcellular localization, and target selection of CDK1. Its destruction is essential for the completion of mitosis. Overexpression of human cyclin B1 has been linked to aggressive tumor behavior and is frequently observed in various cancers.
CDK1 plays a key role in driving the M-phase in both meiosis and mitosis. It becomes highly active at the start of the M-phase and becomes inactive at the end. CDK1 controls various important events in the cell, such as DNA replication, segregation, and repair, as well as mRNA transcription and cell morphogenesis. Additionally, it regulates ribosome assembly by targeting specific ribosomal proteins.
CDK1 plays a key role in driving the M-phase in both meiosis and mitosis. It becomes highly active at the start of the M-phase and becomes inactive at the end. CDK1 controls various important events in the cell, such as DNA replication, segregation, and repair, as well as mRNA transcription and cell morphogenesis. Additionally, it regulates ribosome assembly by targeting specific ribosomal proteins.
包裝
也提供250 µg的规格-订购250 µg的规格时，请致电咨询价格和供货情况，参考目录号14-450M。
品質
通过组蛋白H1底物的磷酸化进行常规评估
外觀
分别使用Ni2+/NTA-琼脂糖和GST-琼脂糖进行纯化。然后使用CAK活化cdk1，并通过Q Sepharose™和Ni2+/NTA-琼脂糖再纯化
儲存和穩定性
在-70°C下可保存6个月
其他說明
比活相关数据，请参阅检验报告以获取该酶的单独批次。
法律資訊
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Lin Deng et al.
Molecular cell, 73(5), 915-929 (2019-03-09)
DNA replication errors generate complex chromosomal rearrangements and thereby contribute to tumorigenesis and other human diseases. One mechanism that triggers these errors is mitotic entry before the completion of DNA replication. To address how mitosis might affect DNA replication, we
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