推荐产品
包裝
vial of 6 mL
品質等級
100
200
儲存溫度
−20°C
相关类别
應用
样品缓冲液，Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。
生化／生理作用
Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液（1:1）混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性，并给每个蛋白质和亚基带来整体的负电荷，以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料，也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。
其他說明
含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。
數量
推荐使用方法：将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。
相關產品
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
儲存類別代碼
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
其他客户在看
Amanda M Gonzales et al.
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The Journal of Neuroscience, 34, 9867-9879 (2014)
Dana E Nuti et al.
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GADD45A and EPB41 as tumor suppressor genes in meningioma pathogenesis.
Piaskowski S, et al.
Cancer Genetics and Cytogenetics, 162, 63-67 (2005)
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XPF endonuclease is one of the most important DNA repair proteins. Encoded by XPF/ERCC4, XPF provides the enzymatic activity of XPF-ERCC1 heterodimer, an endonuclease that incises at the 5' side of various DNA lesions. XPF is essential for nucleotide excision
实验方案
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
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