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相关类别
應用
样品缓冲液，Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。
生化／生理作用
Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液（1:1）混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性，并给每个蛋白质和亚基带来整体的负电荷，以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料，也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。
其他說明
含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。
數量
推荐使用方法：将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
儲存類別代碼
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
其他客户在看
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实验方案
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
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