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無菌
0.2 μm filtered
形狀
liquid
應用
diagnostic assay manufacturing
儲存溫度
2-8°C
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一般說明
Tris-甘氨酸-SDS(TGS)电泳缓冲液是蛋白十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)最常用的缓冲液。TGS 通常在阳极和阴极同时使用。如使用微型垂直凝胶电泳仪,建议电泳电压为150伏。
應用
Tris-甘氨酸-SDS缓冲液10x浓缩液在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中用作电泳缓冲液。进行蛋白印迹时,也可在TGS中加入5%脱脂奶粉作为封闭膜。TGS通常同时用作阳极缓冲液和阴极缓冲液。如使用微型垂直凝胶电泳仪,建议电泳电压为150伏。
重構
将 10× TGS 缓冲液稀释为 1× 电泳缓冲液,包含 25mM Tris,192mM 甘氨酸和 0.1% SDS,pH 约为 8.6。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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