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Merck
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主要文件

T1165

Sigma-Aldrich

10× Tris-三(羟甲基)甲基甘氨酸-SDS 缓冲液

别名:

10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

無菌

0.2 μm filtered

品質等級

形狀

liquid

pH值

8.05-8.30 (25 °C in water, high purity, 1:10)

應用

diagnostic assay manufacturing

儲存溫度

2-8°C

一般說明

Tris-Tricine-SDS(TTS)电泳缓冲液是使用Schagger和von Jagow方法对蛋白质进行SDS-聚丙烯酰胺凝胶电泳的阴极(上部储库)缓冲液。Schagger和von Jagow方法专用于分离小分子量蛋白质。它与Laemmli方法的不同之处在于使用tricine取代了甘氨酸,并且凝胶含有1M Tris-HCl,pH 8.45而不是0.375M Tris-HCl,pH8.9。将TTS缓冲液稀释10倍将获得含有100mM Tris、100mM tricine和0.1%SDS的1X阴极缓冲液。推荐电泳条件是125伏。

應用

Tris-Tricine-SDS缓冲液10×浓缩液适合用作支气管肺泡灌洗液(BALF)的聚丙烯酰胺分离的运行缓冲液。

其他說明

1M Tris,1M 三(羟甲基)甲基甘氨酸,1% (w/v) SDS,pH 约为 8.2。

分析報告

经测试用作阴极缓冲液,用于 Tris-三(羟甲基)甲基甘氨酸凝胶电泳分离低分子量的蛋白质。

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Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine
Rudolph C, et al.
Biochim. Biophys. Acta Gen. Subj., 1573(1), 75-83 (2002)
Bonnie J Berry et al.
ACS chemical neuroscience, 9(7), 1693-1701 (2018-05-11)
Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Rachel E Gerver et al.
Analytical chemistry, 86(21), 10625-10632 (2014-10-01)
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

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