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無菌
0.2 μm filtered
品質等級
形狀
liquid
pH值
8.05-8.30 (25 °C in water, high purity, 1:10)
應用
diagnostic assay manufacturing
儲存溫度
2-8°C
一般說明
Tris-Tricine-SDS(TTS)电泳缓冲液是使用Schagger和von Jagow方法对蛋白质进行SDS-聚丙烯酰胺凝胶电泳的阴极(上部储库)缓冲液。Schagger和von Jagow方法专用于分离小分子量蛋白质。它与Laemmli方法的不同之处在于使用tricine取代了甘氨酸,并且凝胶含有1M Tris-HCl,pH 8.45而不是0.375M Tris-HCl,pH8.9。将TTS缓冲液稀释10倍将获得含有100mM Tris、100mM tricine和0.1%SDS的1X阴极缓冲液。推荐电泳条件是125伏。
應用
Tris-Tricine-SDS缓冲液10×浓缩液适合用作支气管肺泡灌洗液(BALF)的聚丙烯酰胺分离的运行缓冲液。
其他說明
1M Tris,1M 三(羟甲基)甲基甘氨酸,1% (w/v) SDS,pH 约为 8.2。
分析報告
经测试用作阴极缓冲液,用于 Tris-三(羟甲基)甲基甘氨酸凝胶电泳分离低分子量的蛋白质。
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