X4500

Xanthine Oxidase from bovine milk

Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

• Sinónimos: Schardinger enzyme, XnOx, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
• Número de CAS: 9002-17-9
• Comisión internacional de enzimas: 1.17.3.2.
• Número CE: 232-657-6
• Número MDL: MFCD00082145
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine milk
• Nivel de calidad: 200
• tpo: Grade III
• Formulario: ammonium sulfate suspension
• actividad específica: ≥0.8 units/mg protein
• técnicas: inhibition assay: suitable
• color: faint brown to dark brown
• Nº de acceso UniProt: A0A3Q1LW91; A0A3Q1MM92; A0A3S5ZPW7
• actividad extraña: uricase ≤0.1%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

enthält auch proteolytische Aktivität

Research area: Cell Cycle

Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Aplicación

Xanthine Oxidase from bovine milk has been used:

• as a source for superoxide generation
• for the degradation of hypoxanthine
• for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method
• as a constituent of assay mixture in xanthine oxidase inhibition assay

Acciones bioquímicas o fisiológicas

Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB), febuxostat and allopurinol. XO and xanthine dehydrogenase (XDH) plays a vital role in the last two steps in the formation of urate. Elevated levels of XO has been observed in the serum of chronic liver disease patients. Therefore, XO can be used as a biomarker for the detection of liver disease.

Xanthine oxidase activity is inhibited by folic acid.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Definición de unidad

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forma física

Suspension in 2.3 M (NH₄)₂SO₄, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate

Nota de preparación

Chromatographically purified

Almacenamiento y estabilidad

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Nota de análisis

Protein determined by biuret

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P304 + P340 + P312 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)