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Merck

X1875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

Sinónimos:

XOD, Xanthine:oxygen oxidoreductase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
1.17.3.2.
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

tpo

Grade I

Nivel de calidad

Formulario

ammonium sulfate suspension

actividad específica

≥0.4 units/mg protein

actividad extraña

uricase ≤0.5%

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

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Descripción general

enthält auch proteolytische Aktivität
Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

Aplicación

Xanthine Oxidase from bovine milk has been used:
  • in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
  • in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
  • as a standard to determine XO activity
  • as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Acciones bioquímicas o fisiológicas

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

Definición de unidad

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forma física

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Nota de análisis

Protein determined by biuret

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Antioxidant properties of propofol and erythropoietin after closed head injury in rats
Ozturk E, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 29, 922-927 (2005)
Methods of Enzymatic Analysis (2012)
Asep I M Ali et al.
Animals : an open access journal from MDPI, 9(5) (2019-05-06)
Given their high nitrogen (N) concentration and low costs, sweet potato vine silage (SPVS) and urea-molasses blocks (UMB) are recommended supplements for tropical regions; therefore, they were investigated in this study. Six heifers were allocated to three diets: the roughage
Xanthine oxidase: isolation, assays of activity, and inhibition
Kostic DA, et al.
Journal of Chemistry, 2015 (2015)
Ya Liu et al.
Frontiers in nutrition, 9, 879354-879354 (2022-05-03)
To promote the normal metabolism of human uric acid, high-performance hyaluronic acid-black rice anthocyanins (HAA) nanocomposite particles were successfully prepared by a simple crosslinking method as a novel xanthine oxidase inhibitor. Its structure and properties were characterized by scanning electron

Artículos

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolos

Enzymatic Assay of Superoxide Dismutase

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