10110434001

Xanthine Oxidase (XOD)

from cow milk

• Sinónimos: XOD
• Comisión internacional de enzimas: 1.1.3.22 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: bovine milk
• Nivel de calidad: 100
• Formulario: suspension
• actividad específica: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• envase: pkg of 1 mL (20 U)
• fabricante / nombre comercial: Roche
• pH óptimo: 8.5-9.0
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Aplicación

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Acciones bioquímicas o fisiológicas

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Calidad

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Secuencia

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Definición de unidad

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Forma física

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Nota de preparación

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash