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S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grado

Molecular Biology
for molecular biology

Formulario

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

actividad extraña

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. de almacenamiento

room temp

Descripción general

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Aplicación

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Características y beneficios

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Componentes

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCK2192
SLCC8348
SLBX2706
SLBW3443
SLBV7125

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A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

Protocolos

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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