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S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinónimos:

Saline-sodium citrate buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

Nivel de calidad

200

formulario

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubilidad

water: 263 mg/mL, clear, colorless

actividad extraña

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. de almacenamiento

room temp

Descripción general

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Aplicación

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Características y beneficios

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Componentes

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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