N4014

Nystatin

powder, suitable for cell culture, BioXtra

• Sinónimos: Fungicidin, Mycostatin
• Número de CAS: 1400-61-9
• Número CE: 215-749-0
• Número MDL: MFCD25541651
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 24897362
• NACRES: NA.76

Propiedades

• product name: Nystatin, powder, γ-irradiated, BioXtra, suitable for cell culture
• Nivel de calidad: 200; 300
• esterilidad: γ-irradiated
• Línea del producto: BioXtra
• formulario: powder
• mol peso: 926.1 g/mol
• técnicas: cell culture | mammalian: suitable
• espectro de actividad antibiótica: fungi; yeast
• Modo de acción: cell membrane | interferes
• temp. de almacenamiento: −20°C
• cadena SMILES: O[C@@H]([C@H](C)[C@H](C)O1)[C@@H](C)/C=C/C=C/CC/C=C/C=C/C=C/C=C/[C@H](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](N)[C@@H]2O)C[C@@]3([H])[C@H](C(O)=O)[C@@H](O)C[C@](O)(O3)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)C[C@@H](O)CC1=O
• InChI: 1S/C47H75NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-36(53)35(52)20-19-31(49)21-32(50)22-33(51)23-39(55)62-29(3)28(2)42(27)56/h5-6,8,10-18,27-38,40-44,46,49-54,56-58,61H,7,9,19-26,48H2,1-4H3,(H,59,60)/b6-5+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28+,29-,30+,31+,32+,33+,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• Clave InChI: VQOXZBDYSJBXMA-QEKUPDCNSA-N

Descripción

Descripción general

Chemical structure: polyene

Aplicación

Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells. This product is recommended for use in cell culture applications at 50 mg/L.

Acciones bioquímicas o fisiológicas

Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol.

Antimicrobial spectrum: Nystatin acts against fungi, yeasts and molds.

Precaución

Nystatin in tissue culuture media is stable at 37°C for three days. It is also stable in moderately alkaline media. Solutions and aqueous suspensions begin to lose activity soon after preparation, with heat, light, and oxygen accelerating the decomposition. Aqueous suspensions are stable for 10 minutes when heating to 100°C at pH 7.

Nota de preparación

Nystatin is soluble at 28 °C in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL), and ethylene glycol (8.75 mg/mL). It is also soluble in DMSO at 5 mg/mL and freely soluble in DMF and formamide. It is not recommended to autoclave or sterile filter solutions of Nystatin. Nystatin is also effective as a suspension, stable at 37 °C for 3 days. Prepare 50 mg/mL stock suspensions in water and store at -20 °C. This product is γ-irradiated from N6261.

Otras notas

Keep container tightly closed in a dry and well-ventilated place.Store under inert gas.Air and light sensitive.Keep in a dry place

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)