L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

• Sinónimos: Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
• Número de CAS: 9054-63-1
• Comisión internacional de enzimas: 3.4.11.2 (BRENDA, IUBMB)
• Número CE: 232-942-5
• Número MDL: MFCD00131501
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: Porcine kidney
• tpo: Type VI-S
• formulario: lyophilized powder
• actividad específica: ≥12 units/mg protein (biuret)
• mol peso: 320 kDa
• composición: Protein, 30-70%
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Aplicación

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.

Acciones bioquímicas o fisiológicas

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Definición de unidad

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Forma física

Contains primarily phosphate buffer salts, pH 7.0

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H334,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)